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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   C13orf3 Antibody (Center)   

C13orf3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - C13orf3 Antibody (Center) AP8979c
    Western blot analysis of C13orf3 Antibody (Center) (Cat. #AP8979c) in Hela, 293 cell line lysates (35ug/lane). C13orf3 (arrow) was detected using the purified Pab.
  • IHC-P - C13orf3 Antibody (Center) AP8979c
    C13orf3 Antibody (Center) (Cat. #AP8979c) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - C13orf3 Antibody (Center) AP8979c
    C13orf3 Antibody (Center) (Cat. #AP8979c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q8IX90
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 46359 Da
Antigen Region 226-253 aa
Additional Information
Gene ID 221150
Other Names Spindle and kinetochore-associated protein 3, SKA3, C13orf3, RAMA1
Target/Specificity This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 amino acids from the Central region of human C13orf3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC13orf3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SKA3
Synonyms C13orf3, RAMA1
Function Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:19289083, PubMed:19360002, PubMed:23085020). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083, PubMed:19360002). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:19289083). In the complex, it mediates the microtubule-stimulated oligomerization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).
Cellular Location Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner
Research Areas
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Background

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.

References

Rush,J., et.al., Nat. Biotechnol. 23 (1), 94-101 (2005)

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Cat# AP8979c
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(40 western blots)
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