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C15orf2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - C15orf2 Antibody (Center) AP8982c
    All lanes : Anti-C15orf2 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - C15orf2 Antibody (Center) AP8982c
    Formalin-fixed and paraffin-embedded human testis tissue reacted with C15orf2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - C15orf2 Antibody (Center) AP8982c
    C15orf2 Antibody (Center) (Cat. #AP8982c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q9NZP6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 120954 Da
Antigen Region 348-377 aa
Additional Information
Gene ID 23742
Other Names Nuclear pore-associated protein 1, NPAP1, C15orf2
Target/Specificity This C15orf2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-377 amino acids from the Central region of human C15orf2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC15orf2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPAP1
Synonyms C15orf2
Function May be involved in spermatogenesis.
Cellular Location Nucleus, nucleoplasm. Nucleus inner membrane. Note=Colocalizes with the NPC and nuclear lamins at the nuclear periphery
Tissue Location Testis-specific in adults. In fetal brain expressed only from the paternal allele.
Citations ( 0 )

Background

C15orf2 may be involved in spermatogenesis.

References

Farber,C., et.al., Genomics 65 (2), 174-183 (2000)

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$ 365.00
$ 140.00
Cat# AP8982c
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