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NKX3-1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NKX3-1 Antibody (Center) AP8996C
    Anti-NKX3-1 Antibody (Center) at 1:8000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NKX3-1 Antibody (Center) AP8996C
    Western blot analysis of NKX3-1 Antibody (Center) (Cat. #AP8996c) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.
  • IHC-P - NKX3-1 Antibody (Center) AP8996C
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - NKX3-1 Antibody (Center) AP8996C
    NKX3-1 Antibody (Center) (Cat. #AP8996c) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q99801
Other Accession P97436
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 26350 Da
Antigen Region 3-1 aa
Additional Information
Gene ID 4824
Other Names Homeobox protein Nkx-31, Homeobox protein NK-3 homolog A, NKX3-1, NKX31, NKX3A
Target/Specificity This NKX3-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human NKX3-1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKX3-1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX3-1
Synonyms NKX3.1, NKX3A
Function Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:11137288}
Tissue Location Highly expressed in the prostate and, at a lower level, in the testis.
Citations (0)

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Background

NKX3-1 is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. It play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.

References

Voeller,H.J., et.al., Cancer Res. 57 (20), 4455-4459 (1997)

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$ 295.00
$ 99.00
Cat# AP8996C
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Quantity:
(40 western blots)
Availability: In Stock
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