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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CLASP2 Antibody (Y1019)   

CLASP2 Antibody (Y1019)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CLASP2 Antibody (Y1019) AP9035a
    Western blot analysis of CLASP (arrow) using rabbit polyclonal CLASP Antibody (Y1019) (Cat. #AP9035a). 293 cell lysates transfected with the ACADL gene (Lane 1) and with the GFP-CLASP (Lane 2).
  • WB - CLASP2 Antibody (Y1019) AP9035a
    Western blot analysis of CLASP2 Antibody (Y1019) (Cat. #AP9035a) in mouse spleen tissue lysates (35ug/lane). CLASP2 (arrow) was detected using the purified Pab.
  • FC - CLASP2 Antibody (Y1019) AP9035a
    CLASP2 Antibody (Y1019) (Cat. #AP9035a) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O75122
Other Accession Q99JD4, Q8BRT1
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 141133 Da
Antigen Region 998-1026 aa
Additional Information
Gene ID 23122
Other Names CLIP-associating protein 2, Cytoplasmic linker-associated protein 2, Protein Orbit homolog 2, hOrbit2, CLASP2, KIAA0627
Target/Specificity This CLASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 998-1026 amino acids from human CLASP2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLASP2 Antibody (Y1019) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLASP2
Synonyms KIAA0627
Function Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2- dependent stabilization of microtubules at the cell cortex.
Cellular Location Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle. Golgi apparatus {ECO:0000250|UniProtKB:Q8BRT1}. Golgi apparatus, trans-Golgi network. Cell membrane. Cell projection, ruffle membrane. Note=Localizes to microtubule plus ends (PubMed:15631994). Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514). In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.
Tissue Location Brain-specific.
Research Areas
Citations (0)

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Background

CLASP2 is microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

References

Inkster,B., et.al., Neuroimage (2010) In press.

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Cat# AP9035a
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(40 western blots)
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