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CARD6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - CARD6 Antibody (Center) AP9043c
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center)(Cat#AP9043c). AP9043c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - CARD6 Antibody (Center) AP9043c
    Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody (Center)(Cat#AP9043c). AP9043c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - CARD6 Antibody (Center) AP9043c
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center)(Cat#AP9043c). AP9043c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - CARD6 Antibody (Center) AP9043c
    Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody (Center)(Cat. #AP9043c). AP9043c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - CARD6 Antibody (Center) AP9043c
    Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody (Center)(Cat. #AP9043c). AP9043c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - CARD6 Antibody (Center) AP9043c
    CARD6 Antibody (Center) (Cat.# AP9043c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CARD6 antibody detected the CARD6 protein (arrow).
  • WB - CARD6 Antibody (Center) AP9043c
    Western blot analysis of CARD6 Antibody (Center) (Cat. #AP9043c) in MDA-MB231 cell line lysates (35ug/lane). CARD6 (arrow) was detected using the purified Pab.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9BX69
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 116468 Da
Antigen Region 755-787 aa
Additional Information
Gene ID 84674
Other Names Caspase recruitment domain-containing protein 6, CARD6
Target/Specificity This CARD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CARD6.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCARD6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CARD6
Function May be involved in apoptosis.
Research Areas
Citations (0)

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Background

CARD6 is a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB.

References

Kim,S.S., et.al., Pathology 42 (1), 50-53 (2010)
Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)

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$ 295.00
$ 99.00
Cat# AP9043c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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