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BPI Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BPI Antibody (Center) AP9060c
    Western blot analysis of BPI Antibody (Center) (Cat. #AP9060c) in HL-60 cell line lysates (35ug/lane). BPI (arrow) was detected using the purified Pab.
    detail
  • IHC-P - BPI Antibody (Center) AP9060c
    Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - BPI Antibody (Center) AP9060c
    BPI Antibody (Center) (Cat. #AP9060c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P17213
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53900 Da
Antigen Region 293-324 aa
Additional Information
Gene ID 671
Other Names Bactericidal permeability-increasing protein, BPI, CAP 57, BPI
Target/Specificity This BPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-324 amino acids from the Central region of human BPI.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBPI Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BPI
Function The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Cellular Location Secreted. Cytoplasmic granule membrane Note=Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.
Tissue Location Restricted to cells of the myeloid series.
Research Areas
Citations (0)
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Background

BPI encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has bactericidal activity on gram-negative organisms.

References

Davila,S., et.al., Genes Immun. (2010) In press
Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)

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$ 365.00
$ 140.00
Cat# AP9060c
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