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UTY Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - UTY Antibody (C-term) AP9074b
    Confocal immunofluorescent analysis of UTY Antibody (C-term)(Cat#AP9074b) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - UTY Antibody (C-term) AP9074b
    All lanes : Anti-UTY_HUMAN at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: U266B1 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 150 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - UTY Antibody (C-term) AP9074b
    UTY Antibody (C-term) (Cat. #AP9074b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession O14607
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 149548 Da
Antigen Region 1247-1276 aa
Additional Information
Gene ID 7404
Other Names Histone demethylase UTY, 11411-, Ubiquitously-transcribed TPR protein on the Y chromosome, Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein, UTY, KDM6C
Target/Specificity This UTY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1247-1276 amino acids from the C-terminal region of human UTY.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUTY Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UTY
Synonyms KDM6C
Function Male-specific histone demethylase that catalyzes trimethylated 'Lys-27' (H3K27me3) demethylation in histone H3. Has relatively low lysine demethylase activity.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

UTY encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. This protein is a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts.

References

Russo,P., et.al., Arterioscler. Thromb. Vasc. Biol. 28 (8), 1569-1574 (2008)
Gerrard,D.T. et.al., Mol. Biol. Evol. 22 (6), 1423-1432 (2005)

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$ 365.00
$ 140.00
Cat# AP9074b
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