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ACR Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ACR Antibody (C-term) AP9079b
    Western blot analysis of ACR Antibody (C-term) (Cat. #AP9079b) in Jurkat cell line lysates (35ug/lane). ACR (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ACR Antibody (C-term) AP9079b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with ACR Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P10323
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45847 Da
Antigen Region 387-416 aa
Additional Information
Gene ID 49
Other Names Acrosin, Acrosin light chain, Acrosin heavy chain, ACR, ACRS
Target/Specificity This ACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-416 amino acids from the C-terminal region of human ACR.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACR Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACR
Synonyms ACRS
Function Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
Research Areas
Citations (0)
citation

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Background

ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

References

Dube,C., et.al., J. Androl. 26 (4), 519-528 (2005)
Furlong,L.I., et.al., Fertil. Steril. 83 (6), 1791-1796 (2005)

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$ 365.00
$ 140.00
Cat# AP9079b
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