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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GCLM Antibody (C-term)   

GCLM Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - GCLM Antibody (C-term) AP9102B
    AP9102B staining GCLM in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - GCLM Antibody (C-term) AP9102B
    AP9102B staining GCLM in human cervical carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - GCLM Antibody (C-term) AP9102B
    Anti-GCLM Antibody (C-term) at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GCLM Antibody (C-term) AP9102B
    Western blot analysis of GCLM Antibody (C-term) (Cat. #AP9102b) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.
  • FC - GCLM Antibody (C-term) AP9102B
    GCLM Antibody (C-term) (Cat. #AP9102b) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - GCLM Antibody (C-term) AP9102B
    Western blot analysis of GCLM (arrow) using rabbit polyclonal GCLM Antibody (C-term) (Cat. #AP9102b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCLM gene.
  • IF - GCLM Antibody (C-term) AP9102B
    Confocal immunofluorescent analysis of GCLM Antibody (C-term)(Cat#AP9102b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, FC, IF, E
Primary Accession P48507
Other Accession O09172
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30727 Da
Antigen Region 246-274 aa
Additional Information
Gene ID 2730
Other Names Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GCLM, GLCLR
Target/Specificity This GCLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the C-terminal region of human GCLM.
Dilution IHC-P~~1:25
WB~~1:1000
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGCLM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCLM
Synonyms GLCLR
Tissue Location In all tissues examined. Highest levels in skeletal muscle
Research Areas
Citations ( 0 )

Background

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

References

Moyer,A.M., et.al., Cancer Epidemiol. Biomarkers Prev. 19 (3), 811-821 (2010) Engstrom,K.S., et.al., Mutat. Res. 683 (1-2), 98-105 (2010)

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$ 295.00
$ 99.00
Cat# AP9102B
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(40 western blots)
Availability: In Stock
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