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IL10 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IL10 Antibody (Center) AP9112C
    Western blot analysis of IL10 Antibody (Center) (Cat. #AP9112c) in MDA-MB435 cell line lysates (35ug/lane). IL10 (arrow) was detected using the purified Pab.
  • IHC-P - IL10 Antibody (Center) AP9112C
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - IL10 Antibody (Center) AP9112C
    IL10 Antibody (Center) (Cat. #AP9112c) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22301
Other Accession P79338, P03180
Reactivity Human
Predicted EBV, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20517 Da
Antigen Region 27-53 aa
Additional Information
Gene ID 3586
Other Names Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Target/Specificity This IL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from the Central region of human IL10.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL10
Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Cellular Location Secreted.
Tissue Location Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types
Research Areas
Citations (0)

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The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.


Trajkov,D.,, Indian J Tuberc 56 (3), 117-131 (2009)
Kim,J.M.,, J. Immunol. 148 (11), 3618-3623 (1992)

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Cat# AP9112C
(40 western blots)
Availability: Inquire
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