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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TIMP3 Antibody (Center)   

TIMP3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TIMP3 Antibody (Center) AP9124c
    Western blot analysis of TIMP3 Antibody (Center) (Cat. #AP9124c) in HepG2 cell line lysates (35ug/lane). TIMP3 (arrow) was detected using the purified Pab.
  • WB - TIMP3 Antibody (Center) AP9124c
    Western blot analysis of TIMP3 Antibody (Center) Pab (Cat. #AP9124c) pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab.
  • IHC-P - TIMP3 Antibody (Center) AP9124c
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - TIMP3 Antibody (Center) AP9124c
    TIMP3 Antibody (Center) (Cat. #AP9124c) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35625
Other Accession P48032, O97590, P39876, P61269, P79121, Q9TUL9
Reactivity Human, Mouse
Predicted Bovine, Monkey, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24145 Da
Antigen Region 113-139 aa
Additional Information
Gene ID 7078
Other Names Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3
Target/Specificity This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTIMP3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TIMP3
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)

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TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).


Jacobson,S.G.,, Nat. Genet. 11 (1), 27-32 (1995) Wick,M.,, Biochem. J. 311 (PT 2), 549-554 (1995)

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Cat# AP9124c
(40 western blots)
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