|Application ||WB, E|
|Calculated MW||35214 Da|
|Antigen Region||288-315 aa|
|Other Names||Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6|
|Target/Specificity||This CD47 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-315 amino acids from the C-terminal region of human CD47.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD47 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain|
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Provided below are standard protocols that you may find useful for product applications.
CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.
Hatherley D.,et.al., Mol. Cell 31:266-277(2008).
Wollscheid B., et.al., Nat. Biotechnol. 27:378-386(2009).
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