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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   CD47 Antibody (C-term)   

CD47 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD47 Antibody (C-term) AP9137b
    Western blot analysis of CD47 Antibody (C-term) (Cat. #AP9137b) in T47D cell line lysates (35ug/lane). CD47 (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q08722
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 288-315 aa
Additional Information
Other Names Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6
Target/Specificity This CD47 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-315 amino acids from the C-terminal region of human CD47.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD47 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD47
Synonyms MER6
Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain EMBL; X69398; CAA49196.1; -; mRNA EMBL; Z25521; CAA80977.1; -; mRNA EMBL; BT006907; AAP35553.1; -; mRNA EMBL; AK289813; BAF82502.1; -; mRNA EMBL; CH471052; EAW79733.1; -; Genomic_DNA EMBL; CH471052; EAW79734.1; -; Genomic_DNA EMBL; BC010016; AAH10016.1; -; mRNA EMBL; BC012884; AAH12884.1; -; mRNA EMBL; BC037306; AAH37306.1; -; mRNA CCDS; CCDS43125.1; -. [Q08722-3] CCDS; CCDS43126.1; -. [Q08722-1] PIR; A48997; A48997 RefSeq; NP_001768.1; NM_001777.3. [Q08722-1] RefSeq; NP_942088.1; NM_198793.2. [Q08722-3] UniGene; Hs.446414; - PDB; 2JJS; X-ray; 1.85 A; C/D=20-136 PDB; 2JJT; X-ray; 2.30 A; C/D=20-136 PDB; 2VSC; X-ray; 1.90 A; A/B/C/D=20-136 PDB; 4CMM; X-ray; 1.92 A; B=20-136 PDB; 4KJY; X-ray; 1.93 A; A/C=19-135 PDB; 5IWL; X-ray; 2.80 A; C/D=20-132 PDB; 5TZ2; X-ray; 2.30 A; C=19-141 PDB; 5TZT; X-ray; 2.89 A; C/D=20-141 PDB; 5TZU; X-ray; 2.10 A; C=19-141 PDBsum; 2JJS; - PDBsum; 2JJT; - PDBsum; 2VSC; - PDBsum; 4CMM; - PDBsum; 4KJY; - PDBsum; 5IWL; - PDBsum; 5TZ2; - PDBsum; 5TZT; - PDBsum; 5TZU; - ProteinModelPortal; Q08722; - SMR; Q08722; - BioGrid; 107399; 26 CORUM; Q08722; - DIP; DIP-39948N; - IntAct; Q08722; 8 MINT; Q08722; - STRING; 9606.ENSP00000355361; - ChEMBL; CHEMBL3714583; - TCDB; 1.N.1.1.1; the osteoclast fusion complex (ofc) family iPTMnet; Q08722; - PhosphoSitePlus; Q08722; - SwissPalm; Q08722; - BioMuta; CD47; - DMDM; 1171879; - EPD; Q08722; - MaxQB; Q08722; - PaxDb; Q08722; - PeptideAtlas; Q08722; - PRIDE; Q08722; - ProteomicsDB; 58643; - ProteomicsDB; 58644; -. [Q08722-2] ProteomicsDB; 58645; -. [Q08722-3] ProteomicsDB; 58646; -. [Q08722-4] DNASU; 961; - Ensembl; ENST00000355354; ENSP00000347512; ENSG00000196776. [Q08722-3] Ensembl; ENST00000361309; ENSP00000355361; ENSG00000196776. [Q08722-1] GeneID; 961; - KEGG; hsa:961; - UCSC; uc003dwt.2; human. [Q08722-1] CTD; 961; - DisGeNET; 961; - EuPathDB; HostDB:ENSG00000196776.14; - GeneCards; CD47; - HGNC; HGNC:1682; CD47 HPA; CAB016055; - HPA; HPA044659; - MIM; 601028; gene neXtProt; NX_Q08722; - OpenTargets; ENSG00000196776; - PharmGKB; PA26222; - eggNOG; ENOG410IJTS; Eukaryota eggNOG; ENOG41113GI; LUCA GeneTree; ENSGT00390000007697; - HOGENOM; HOG000013020; - HOVERGEN; HBG003808; - InParanoid; Q08722; - KO; K06266; - OMA; VYMKFVA; - OrthoDB; EOG091G0HUR; - PhylomeDB; Q08722; - TreeFam; TF336026; - Reactome; R-HSA-202733; Cell surface interactions at the vascular wall Reactome; R-HSA-216083; Integrin cell surface interactions Reactome; R-HSA-391160; Signal regulatory protein family interactions Reactome; R-HSA-6798695; Neutrophil degranulation SIGNOR; Q08722; - ChiTaRS; CD47; human EvolutionaryTrace; Q08722; - GeneWiki; CD47; - GenomeRNAi; 961; - PRO; PR:Q08722; - Proteomes; UP000005640; Chromosome 3 Bgee; ENSG00000196776; - CleanEx; HS_CD47; - ExpressionAtlas; Q08722; baseline and differential Genevisible; Q08722; HS GO; GO:0070062; C:extracellular exosome; IEA:Ensembl GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc GO; GO:0005886; C:plasma membrane; TAS:Reactome GO; GO:0035579; C:specific granule membrane; TAS:Reactome GO; GO:0070821; C:tertiary granule membrane; TAS:Reactome GO; GO:0070053; F:thrombospondin receptor activity; IPI:BHF-UCL GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW GO; GO:0030198; P:extracellular matrix organization; TAS:Reactome GO; GO:0007229; P:integrin-mediated signaling pathway; TAS:ProtInc GO; GO:0050900; P:leukocyte migration; TAS:Reactome GO; GO:0043312; P:neutrophil degranulation; TAS:Reactome GO; GO:0008228; P:opsonization; IEA:Ensembl GO; GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB GO; GO:0022409; P:positive regulation of cell-cell adhesion; IDA:UniProtKB GO; GO:0050729; P:positive regulation of inflammatory response; IEA:Ensembl GO; GO:0050766; P:positive regulation of phagocytosis; IEA:Ensembl GO; GO:0050870; P:positive regulation of T cell activation; IDA:UniProtKB GO; GO:0009617; P:response to bacterium; IEA:Ensembl CDD; cd16090; IgV_CD47; 1 Gene3D; 2.60.40.10; -; 1 InterPro; IPR006704; CD47 InterPro; IPR013147; CD47_TM InterPro; IPR013270; CD47_Vset InterPro; IPR007110; Ig-like_dom InterPro; IPR036179; Ig-like_dom_sf InterPro; IPR013783; Ig-like_fold InterPro; IPR037805; IgV_CD47 PANTHER; PTHR10613; PTHR10613; 1 Pfam; PF04549; CD47; 1 Pfam; PF08204; V-set_CD47; 1 SUPFAM; SSF48726; SSF48726; 1 PROSITE; PS50835; IG_LIKE; 1 1: Evidence at protein level; 3D-structure; Alternative splicing; Cell adhesion; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Phosphoprotein; Pyrrolidone carboxylic acid; Reference proteome; Signal; Transmembrane; Transmembrane helix SIGNAL 1 18 CHAIN 19 323 Leukocyte surface antigen CD47 /FTId=PRO_0000014880 TOPO_DOM 19 141 Extracellular. TRANSMEM 142 162 Helical. TOPO_DOM 163 176 Cytoplasmic. TRANSMEM 177 197 Helical. TOPO_DOM 198 207 Extracellular. TRANSMEM 208 228 Helical. TOPO_DOM 229 235 Cytoplasmic. TRANSMEM 236 256 Helical. TOPO_DOM 257 268 Extracellular. TRANSMEM 269 289 Helical. TOPO_DOM 290 323 Cytoplasmic. DOMAIN 19 127 Ig-like V-type MOD_RES 19 19 Pyrrolidone carboxylic acid MOD_RES 89 89 Phosphoserine {ECO:0000250|UniProtKB:P97829} CARBOHYD 23 23 N-linked (GlcNAc...) asparagine CARBOHYD 34 34 N-linked (GlcNAc...) asparagine CARBOHYD 50 50 N-linked (GlcNAc...) asparagine CARBOHYD 73 73 N-linked (GlcNAc...) asparagine CARBOHYD 111 111 N-linked (GlcNAc...) asparagine CARBOHYD 206 206 N-linked (GlcNAc...) asparagine DISULFID 33 263 DISULFID 41 114 VAR_SEQ 293 323 Missing (in isoform OA3-293) /FTId=VSP_002535 VAR_SEQ 304 305 KA -> NN (in isoform OA3-305) {ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:7691831, ECO:0000303|Ref.3} /FTId=VSP_002536 VAR_SEQ 306 323 Missing (in isoform OA3-305) {ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:7691831, ECO:0000303|Ref.3} /FTId=VSP_002537 VAR_SEQ 312 323 Missing (in isoform OA3-312) /FTId=VSP_002538 STRAND 26 30 {ECO:0000244|PDB:2JJS} STRAND 35 39 {ECO:0000244|PDB:2JJS} STRAND 42 45 {ECO:0000244|PDB:2JJS} HELIX 51 53 {ECO:0000244|PDB:2JJS} STRAND 54 60 {ECO:0000244|PDB:2JJS} STRAND 63 69 {ECO:0000244|PDB:2JJS} HELIX 70 72 {ECO:0000244|PDB:2JJS} STRAND 74 76 {ECO:0000244|PDB:2JJS} HELIX 79 81 {ECO:0000244|PDB:2JJS} HELIX 88 93 {ECO:0000244|PDB:2JJS} STRAND 98 101 {ECO:0000244|PDB:2JJS} HELIX 102 106 {ECO:0000244|PDB:2JJS} STRAND 110 118 {ECO:0000244|PDB:2JJS} STRAND 121 131 {ECO:0000244|PDB:2JJS} SEQUENCE 323 AA; 35214 MW; 5D20730A2632D550 CRC64; MWPLVAALLL GSACCGSAQL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN TTEVYVKWKF KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM DKSDAVSHTG NYTCEVTELT REGETIIELK YRVVSWFSPN ENILIVIFPI FAILLFWGQF GIKTLKYRSG GMDEKTIALL VAGLVITVIV IVGAILFVPG EYSLKNATGL GLIVTSTGIL ILLHYYVFST AIGLTSFVIA ILVIQVIAYI LAVVGLSLCI AACIPMHGPL LISGLSILAL AQLLGLVYMK FVASNQKTIQ PPRKAVEEPL NAFKESKGMM NDE
Research Areas
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Background

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

References

Hatherley D.,et.al., Mol. Cell 31:266-277(2008).
Wollscheid B., et.al., Nat. Biotechnol. 27:378-386(2009).

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Cat# AP9137b
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