|Application ||WB, FC, E|
|Calculated MW||54723 Da|
|Antigen Region||57-84 aa|
|Other Names||Cell division cycle protein 20 homolog, p55CDC, CDC20|
|Target/Specificity||This CDC20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human CDC20.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CDC20 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle pole|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
CDC20 is an activating regulatory factor for the APC/C (anaphase promoting complex/cyclosome). It activates the ubiquitination activity of the APC/C. CDC20 confers a strict destruction-box (D-box) dependence on APC. Levels of CDC20, as well as its binding to APC, peak in mitosis and decrease drastically at early G1.
Ge S., et.al., Cell Cycle 8:167-171(2009).
Gauci S., et.al., Anal. Chem. 81:4493-4501(2009).
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