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ACOT8 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP9144b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP9144b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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ACOT8 Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | O14734 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35914 Da |
ACOT8 Antibody (C-term) - Additional info | |
| Gene ID 10005 | |
| Other Names ACOT8; ACTEIII; PTE1; PTE2; Acyl-coenzyme A thioesterase 8; Acyl-CoA thioesterase 8; Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II | |
| Target/Specificity This ACOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290~319 amino acids from the C-terminal region of human ACOT8. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACOT8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACOT8 Antibody (C-term) - Protein Information | |
| Name ACOT8 | |
| Synonyms ACTEIII, PTE1, PTE2 | |
| Function Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation | |
| Cellular Location Peroxisome. | |
| Tissue Location Detected in a T-cell line (at protein level). Ubiquitous | |
ACOT8 Antibody (C-term) - Related products
AP9144b: ACOT8 Antibody (C-term)
RI10068: ACOT8 predesign siRNA
DC09988: Human ACOT8 cDNA Clone
ACOT8 Antibody (C-term) - Application data
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Western blot analysis of ACOT8 Antibody (C-term) (Cat. #AP9144b) in mouse liver tissue lysates (35ug/lane). ACOT8 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ACOT8 Antibody (C-term) (Cat. #AP9144b) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ACOT8 Antibody (C-term) - Research Areas
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BACKGROUND
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. It may mediate Nef-induced down-regulation of CD4. It may be involved in the metabolic regulation of peroxisome proliferation.
REFERENCES
Choudhary C., et.al., Science 325:834-840(2009). Daub H., et.al., Mol. Cell 31:438-448(2008).
