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>   home   >   Products   >   Primary Antibodies   >   BAHD1 Antibody (C-term)   

BAHD1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BAHD1 Antibody (C-term) AP9159b
    Western blot analysis of BAHD1 Antibody (C-term) (Cat. #AP9159b) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.
  • IHC-P - BAHD1 Antibody (C-term) AP9159b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - BAHD1 Antibody (C-term) AP9159b
    BAHD1 Antibody (C-term) (Cat. #AP9159b) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q8TBE0
Other Accession Q497V6
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 84652 Da
Antigen Region 750-777 aa
Additional Information
Gene ID 22893
Other Names Bromo adjacent homology domain-containing 1 protein, BAH domain-containing protein 1, BAHD1, KIAA0945
Target/Specificity This BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 750-777 amino acids from the C-terminal region of human BAHD1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBAHD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BAHD1
Synonyms KIAA0945
Function Heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. May act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. Represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins. At specific stages of Listeria infection, in complex with TRIM28, corepresses interferon- stimulated genes, including IFNL1, IFNL2 and IFNL3.
Cellular Location Nucleus. Chromosome. Note=Localizes to heterochromatin and inactive X chromosome. Colocalizes with histone H3 trimethylated at 'Lys-27' (H3K27me3)
Citations (0)

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Background

BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.

References

Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
Dephoure N., et.al., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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$ 295.00
$ 99.00
Cat# AP9159b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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