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DDX55 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP9162a 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP9162a-ev20 Ev20 size 100 ul In Stock $ 95.00 Add to cart
  • Specification
  • Citiations : 0
  • Reviews
  • Protocols
  • Backgrounds

DDX55 Antibody (N-term) - Product info

ApplicationWB, IHC, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionQ8NHQ9
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW68547 Da

DDX55 Antibody (N-term) - Additional info

Gene ID 57696
Other Names
DDX55; KIAA1595; ATP-dependent RNA helicase DDX55; DEAD box protein 55
Target/Specificity
This DDX55 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-140 amino acids from the N-terminal region of human DDX55.
Dilution
WB~~1:100~500
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
DDX55 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

DDX55 Antibody (N-term) - Protein Information

Name DDX55
Synonyms KIAA1595
Function
Probable ATP-binding RNA helicase.

DDX55 Antibody (N-term) - Related products

AP9162a: DDX55 Antibody (N-term)

AP9162b: DDX55 Antibody (C-term)

RI11461: DDX55 predesign siRNA

DC06965: Human DDX55 cDNA Clone

BP9162a: DDX55 Antibody (N-term) Blocking Peptide

BP9162b: DDX55 Antibody (C-term) Blocking Peptide

DDX55 Antibody (N-term) - Application data

  • Western blot analysis of DDX55 Antibody (N-term) (Cat. #AP9162a) in MDA-MB231 cell line lysates (35ug/lane).DDX55 (arrow) was detected using the purified Pab.

  • DDX55 Antibody (N-term) (Cat. #AP9162a) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX55 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

  • DDX55 Antibody (N-term) (Cat. #AP9162a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

DDX55 Antibody (N-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

DDX55 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

REFERENCES

Andersen, J.S., et al. Nature 433(7021):77-83(2005) Schmid, S.R., et al. Mol. Microbiol. 6(3):283-291(1992)