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>   home   >   Products   >   Primary Antibodies   >   DEK Antibody (C-term)   

DEK Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DEK Antibody (C-term) AP9186b
    Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody (C-term)(Cat. #AP9186b). AP9186b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • IHC-P - DEK Antibody (C-term) AP9186b
    DEK Antibody (C-term) (Cat. #AP9186b) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - DEK Antibody (C-term) AP9186b
    DEK Antibody (C-term) (Cat. #AP9186b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - DEK Antibody (C-term) AP9186b
    Confocal immunofluorescent analysis of DEK Antibody (C-term) (Cat#AP9186b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IF, E
Primary Accession P35659
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42674 Da
Antigen Region 336-364 aa
Additional Information
Gene ID 7913
Other Names Protein DEK, DEK
Target/Specificity This DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human DEK.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDEK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DEK
Function Involved in chromatin organization.
Cellular Location Nucleus. Note=Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei
Tissue Location Ubiquitous. Expressed at relatively high levels
Citations (0)

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Background

DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.

References

Sawatsubashi,S., et.al., Genes Dev. 24 (2), 159-170 (2010)
Khodadoust,M.S., et.al., Cancer Res. 69 (16), 6405-6413 (2009)

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$ 295.00
$ 99.00
Cat# AP9186b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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