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FCGR1B Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FCGR1B Antibody (C-term) AP9197B
    All lanes : Anti-FCGR1B Antibody (C-term) at 1:500 dilution Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - FCGR1B Antibody (C-term) AP9197B
    All lanes : Anti-FCGR1B Antibody (C-term) at 1:500 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - FCGR1B Antibody (C-term) AP9197B
    Immunohistochemical analysis of AP9197b on paraffin-embedded human tonsil tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
    detail
  • IHC-P-Leica - FCGR1B Antibody (C-term) AP9197B
    Immunohistochemical analysis of AP9197b on paraffin-embedded human appendix tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
    detail
  • FC - FCGR1B Antibody (C-term) AP9197B
    FCGR1B Antibody (C-term) (Cat. #AP9197b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P-Leica, E
Primary Accession Q92637
Other Accession A6NKC4, P12314
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 32232 Da
Antigen Region 248-276 aa
Additional Information
Other Names High affinity immunoglobulin gamma Fc receptor IB, IgG Fc receptor IB, Fc-gamma RIB, FcRIB, hFcgammaRIB, FCGR1B, IGFRB
Target/Specificity This FCGR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the C-terminal region of human FCGR1B.
Dilution WB~~1:500
IHC-P-Leica~~1:100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFCGR1B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FCGR1BP (HGNC:3614)
Synonyms FCGR1B, IGFRB
Function May bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. May function in the humoral immune response.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
citation

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Background

FCGR1B may bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. This protein may function in the humoral immune response.

References

Thomas,G., et.al., Nat. Genet. 41 (5), 579-584 (2009)
Kuwano,Y., et.al., Arch. Dermatol. Res. 298 (10), 493-498 (2007)

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$ 365.00
$ 140.00
Cat# AP9197B
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