|Application ||IHC-P, WB, E|
|Calculated MW||75857 Da|
|Antigen Region||597-627 aa|
|Other Names||Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-lipoxygenase 2, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 11311-, ALOX15B|
|Target/Specificity||This ALOX15B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-627 amino acids from the C-terminal region of human ALOX15B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALOX15B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)- HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.|
|Cellular Location||Isoform A: Nucleus. Note=Other isoforms are excluded from the nucleus|
|Tissue Location||Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea.|
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Provided below are standard protocols that you may find useful for product applications.
ALOX15B encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17.
Wecksler,A.T., et.al., Biochemistry 48 (36), 8721-8730 (2009)
Kawakami,Y., et.al, Biosci. Biotechnol. Biochem. 73 (8), 1811-1817 (2009)
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