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ADH6 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ADH6 Antibody (Center) AP9203c
    Western blot analysis of ADH6 Antibody (Center) (Cat. #AP9203c) in Hela cell line lysates (35ug/lane). ADH6 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ADH6 Antibody (Center) AP9203c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ADH6 Antibody (Center) AP9203c
    ADH6 Antibody (Center) (Cat. #AP9203c) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P28332
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 39073 Da
Antigen Region 209-240 aa
Additional Information
Gene ID 130
Other Names Alcohol dehydrogenase 6, ADH6
Target/Specificity This ADH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-240 amino acids from the Central region of human ADH6.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADH6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADH6
Function Alcohol dehydrogenase (PubMed:1755855). Catalyzes the NAD- dependent oxidation of primary alcohols to the corresponding aldehydes (PubMed:1755855). Oxidizes secondary alcohols to the corresponding ketones (By similarity).
Cellular Location Cytoplasm.
Tissue Location Stomach and liver..
Research Areas
Citations (0)
citation

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Background

ADH6 encodes class V alcohol dehydrogenase, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein is expressed in the stomach as well as in the liver, and it contains a glucocorticoid response element upstream of its 5' UTR, which is a steroid hormone receptor binding site.

References

Cui,R., et.al., Gastroenterology 137 (5), 1768-1775 (2009)
Saito,A., et.al., J. Hum. Genet. 54 (6), 317-323 (2009)
Tabakoff,B., et.al., BMC Biol. 7, 70 (2009)

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$ 182.50
$ 70.00
Cat# AP9203c
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