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FOXL1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FOXL1 Antibody (Center) AP9211c
    Western blot analysis of FOXL1 Antibody (Center) (Cat. #AP9211c) in CHO cell line lysates (35ug/lane).FOXL1 (arrow) was detected using the purified Pab;
  • WB - FOXL1 Antibody (Center) AP9211c
    Western blot analysis of FOXL1 Antibody (Center) (Cat. #AP9211c) in mouse kidney tissue lysates (35ug/lane).FOXL1 (arrow) was detected using the purified Pab.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q12952
Other Accession Q64731
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 94-119 aa
Additional Information
Other Names Forkhead box protein L1, Forkhead-related protein FKHL11, Forkhead-related transcription factor 7, FREAC-7, FOXL1, FKHL11, FREAC7
Target/Specificity This FOXL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-119 amino acids from the Central region of human FOXL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXL1
Synonyms FKHL11, FREAC7
Function Transcription factor required for proper proliferation and differentiation in the gastrointestinal epithelium. Target gene of the hedgehog (Hh) signaling pathway via GLI2 AND GLI3 transcription factors (By similarity).
Cellular Location Nucleus. EMBL; AF315075; AAG40312.1; -; Genomic_DNA EMBL; AC009108; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC117226; AAI17227.1; -; mRNA EMBL; U13225; AAA92042.1; -; Genomic_DNA CCDS; CCDS10959.1; - PIR; S51630; S51630 RefSeq; NP_005241.1; NM_005250.2 UniGene; Hs.533830; - ProteinModelPortal; Q12952; - SMR; Q12952; - BioGrid; 108589; 125 IntAct; Q12952; 117 MINT; Q12952; - STRING; 9606.ENSP00000326272; - iPTMnet; Q12952; - PhosphoSitePlus; Q12952; - BioMuta; FOXL1; - DMDM; 13638268; - EPD; Q12952; - PaxDb; Q12952; - PeptideAtlas; Q12952; - PRIDE; Q12952; - ProteomicsDB; 59047; - Ensembl; ENST00000320241; ENSP00000326272; ENSG00000176678 GeneID; 2300; - KEGG; hsa:2300; - UCSC; uc002fjr.4; human CTD; 2300; - DisGeNET; 2300; - EuPathDB; HostDB:ENSG00000176678.5; - GeneCards; FOXL1; - HGNC; HGNC:3817; FOXL1 MIM; 603252; gene neXtProt; NX_Q12952; - OpenTargets; ENSG00000176678; - PharmGKB; PA28234; - eggNOG; KOG2294; Eukaryota eggNOG; COG5025; LUCA GeneTree; ENSGT00760000118904; - HOGENOM; HOG000112632; - HOVERGEN; HBG051650; - InParanoid; Q12952; - KO; K09405; - OMA; PFNASLM; - OrthoDB; EOG091G0HW9; - PhylomeDB; Q12952; - TreeFam; TF316127; - SignaLink; Q12952; - GenomeRNAi; 2300; - PRO; PR:Q12952; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000176678; - CleanEx; HS_FOXL1; - ExpressionAtlas; Q12952; baseline and differential Genevisible; Q12952; HS GO; GO:0005634; C:nucleus; IBA:GO_Central GO; GO:0003677; F:DNA binding; IDA:MGI GO; GO:0003700; F:DNA binding transcription factor activity; ISS:UniProtKB GO; GO:0008301; F:DNA binding, bending; NAS:UniProtKB GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central GO; GO:0030154; P:cell differentiation; IBA:GO_Central GO; GO:0007507; P:heart development; IMP:DFLAT GO; GO:0007275; P:multicellular organism development; NAS:UniProtKB GO; GO:0061146; P:Peyer's patch morphogenesis; IEA:Ensembl GO; GO:0030166; P:proteoglycan biosynthetic process; ISS:UniProtKB GO; GO:0030111; P:regulation of Wnt signaling pathway; ISS:UniProtKB GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW GO; GO:0007495; P:visceral mesoderm-endoderm interaction involved in midgut development; ISS:UniProtKB CDD; cd00059; FH; 1 Gene3D; 1.10.10.10; -; 1 InterPro; IPR001766; Fork_head_dom InterPro; IPR018122; TF_fork_head_CS_1 InterPro; IPR030456; TF_fork_head_CS_2 InterPro; IPR036388; WH-like_DNA-bd_sf InterPro; IPR036390; WH_DNA-bd_sf Pfam; PF00250; Forkhead; 1 PRINTS; PR00053; FORKHEAD SMART; SM00339; FH; 1 SUPFAM; SSF46785; SSF46785; 1 PROSITE; PS00657; FORK_HEAD_1; 1 PROSITE; PS00658; FORK_HEAD_2; 1 PROSITE; PS50039; FORK_HEAD_3; 1 2: Evidence at transcript level; Complete proteome; DNA-binding; Nucleus; Reference proteome; Transcription; Transcription regulation CHAIN 1 345 Forkhead box protein L1 /FTId=PRO_0000091859 DNA_BIND 48 139 Fork-head. {ECO:0000255|PROSITE- ProRule:PRU00089} SEQUENCE 345 AA; 36490 MW; 015D86B57DF3BBC6 CRC64; MSHLFDPRLP ALAASPMLYL YGPERPGLPL AFAPAAALAA SGRAETPQKP PYSYIALIAM AIQDAPEQRV TLNGIYQFIM DRFPFYHDNR QGWQNSIRHN LSLNDCFVKV PREKGRPGKG SYWTLDPRCL DMFENGNYRR RKRKPKPGPG APEAKRPRAE THQRSAEAQP EAGSGAGGSG PAISRLQAAP AGPSPLLDGP SPPAPLHWPG TASPNEDAGD AAQGAAAVAV GQAARTGDGP GSPLRPASRS SPKSSDKSKS FSIDSILAGK QGQKPPSGDE LLGGAKPGPG GRLGASLLAA SSSLRPPFNA SLMLDPHVQG GFYQLGIPFL SYFPLQVPDT VLHFQ
Citations (0)

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Background

The forkhead domain is a 100-amino acid monomeric DNA binding motif originally identified as a region of homology between the Drosophila forkhead protein and rat HNF3. Pierrou et al. (1994) identified 7 human genes containing forkhead domains and designated them forkhead related activators (FREAC) 1 through 7.

References

Rivadeneira,F., et.al., Nat. Genet. 41 (11), 1199-1206 (2009)
Ferrell,R.E., et.al., Lymphat Res Biol 6 (2), 69-76 (2008)
Hassel,S., et.al., Proteomics 4 (5), 1346-1358 (2004)

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Cat# AP9211c
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