|Application ||WB, IHC-P, IF, E|
|Other Accession||P41516, Q01320, P41515|
|Predicted||Hamster, Mouse, Rat|
|Calculated MW||174385 Da|
|Antigen Region||1167-1196 aa|
|Other Names||DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2|
|Target/Specificity||This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TOP2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).|
|Cellular Location||Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm|
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Provided below are standard protocols that you may find useful for product applications.
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Kawamura,R., et.al, J. Cell Biol. 188 (5), 653-663 (2010)
Alchanati,I., et.al, PLoS ONE 4 (12), E8104 (2009)
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