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TOP2A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TOP2A Antibody (C-term) AP9248B
    All lanes : Anti-TOP2A Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 174 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TOP2A Antibody (C-term) AP9248B
    Western blot analysis of TOP2A Antibody (C-term) (Cat. #AP9248b) in A375 cell line lysates (35ug/lane). TOP2A (arrow) was detected using the purified Pab.
  • IHC-P - TOP2A Antibody (C-term) AP9248B
    Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - TOP2A Antibody (C-term) AP9248B
    Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (Cat#AP9248b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11388
Other Accession P41516, Q01320, P41515
Reactivity Human
Predicted Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 174385 Da
Antigen Region 1167-1196 aa
Additional Information
Gene ID 7153
Other Names DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Target/Specificity This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTOP2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOP2A
Synonyms TOP2
Function Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Cellular Location Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm
Research Areas
Citations (0)

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TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.


Kawamura,R.,, J. Cell Biol. 188 (5), 653-663 (2010)
Alchanati,I.,, PLoS ONE 4 (12), E8104 (2009)

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$ 295.00
$ 99.00
Cat# AP9248B
(40 western blots)
Availability: In Stock
Bulk Size
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