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KCTD12 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KCTD12 Antibody (Center) AP9273c
    Western blot analysis of KCTD12 Antibody (Center) (Cat. #AP9273c) in T47D cell line lysates (35ug/lane). KCTD12 (arrow) was detected using the purified Pab.
  • IHC-P - KCTD12 Antibody (Center) AP9273c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with KCTD12 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - KCTD12 Antibody (Center) AP9273c
    KCTD12 Antibody (Center) (Cat. #AP9273c) flow cytometric analysis of T47D cells (right histogram) compared to a negative control (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96CX2
Other Accession Q6WVG3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 242-269 aa
Additional Information
Other Names BTB/POZ domain-containing protein KCTD12, Pfetin, Predominantly fetal expressed T1 domain, KCTD12, C13orf2, KIAA1778, PFET1
Target/Specificity This KCTD12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the Central region of human KCTD12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKCTD12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KCTD12
Synonyms C13orf2, KIAA1778, PFET1
Function Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).
Cellular Location Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse, postsynaptic cell membrane
Tissue Location Present in a variety of fetal organs, with highest expression levels in the cochlea and brain and, in stark contrast, is detected only at extremely low levels in adult organs, such as brain and lung. EMBL; AF359381; AAQ15187.1; -; mRNA EMBL; BC013764; AAH13764.1; -; mRNA CCDS; CCDS9455.1; - RefSeq; NP_612453.1; NM_138444.3 UniGene; Hs.644125; - ProteinModelPortal; Q96CX2; - SMR; Q96CX2; - BioGrid; 125419; 46 CORUM; Q96CX2; - IntAct; Q96CX2; 18 MINT; Q96CX2; - STRING; 9606.ENSP00000317141; - GuidetoPHARMACOLOGY; 1918; - iPTMnet; Q96CX2; - PhosphoSitePlus; Q96CX2; - BioMuta; KCTD12; - DMDM; 50401124; - REPRODUCTION-2DPAGE; IPI00060715; - EPD; Q96CX2; - MaxQB; Q96CX2; - PaxDb; Q96CX2; - PeptideAtlas; Q96CX2; - PRIDE; Q96CX2; - ProteomicsDB; 76237; - Ensembl; ENST00000377474; ENSP00000366694; ENSG00000178695 GeneID; 115207; - KEGG; hsa:115207; - UCSC; uc010aeu.2; human CTD; 115207; - DisGeNET; 115207; - EuPathDB; HostDB:ENSG00000178695.5; - GeneCards; KCTD12; - H-InvDB; HIX0171820; - HGNC; HGNC:14678; KCTD12 HPA; HPA056987; - HPA; HPA077376; - MIM; 610521; gene neXtProt; NX_Q96CX2; - OpenTargets; ENSG00000178695; - PharmGKB; PA25512; - eggNOG; KOG2723; Eukaryota eggNOG; ENOG410Z155; LUCA GeneTree; ENSGT00760000119013; - HOGENOM; HOG000041197; - HOVERGEN; HBG052218; - InParanoid; Q96CX2; - KO; K21918; - OMA; RDPDHGM; - OrthoDB; EOG091G0P3F; - PhylomeDB; Q96CX2; - TreeFam; TF315332; - ChiTaRS; KCTD12; human GeneWiki; KCTD12; - GenomeRNAi; 115207; - PRO; PR:Q96CX2; - Proteomes; UP000005640; Chromosome 13 Bgee; ENSG00000178695; - CleanEx; HS_KCTD12; - ExpressionAtlas; Q96CX2; baseline and differential Genevisible; Q96CX2; HS GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell GO; GO:0042734; C:presynaptic membrane; IEA:UniProtKB-SubCell GO; GO:0042802; F:identical protein binding; IPI:IntAct GO; GO:0003723; F:RNA binding; HDA:UniProtKB GO; GO:0051260; P:protein homooligomerization; IEA:InterPro InterPro; IPR000210; BTB/POZ_dom InterPro; IPR011333; SKP1/BTB/POZ_sf InterPro; IPR003131; T1-type_BTB Pfam; PF02214; BTB_2; 1 SMART; SM00225; BTB; 1 SUPFAM; SSF54695; SSF54695; 1 1: Evidence at protein level; Acetylation; Cell junction; Cell membrane; Complete proteome; Membrane; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Synapse INIT_MET 1 1 Removed. CHAIN 2 325 BTB/POZ domain-containing protein KCTD12 /FTId=PRO_0000191295 MOD_RES 2 2 N-acetylalanine MOD_RES 119 119 Phosphotyrosine {ECO:0000250|UniProtKB:Q6WVG3} MOD_RES 151 151 Phosphoserine MOD_RES 171 171 Phosphoserine {ECO:0000250|UniProtKB:Q6WVG3} MOD_RES 185 185 Phosphoserine MOD_RES 196 196 Phosphothreonine {ECO:0000250|UniProtKB:Q6WVG3} MOD_RES 200 200 Phosphoserine {ECO:0000250|UniProtKB:Q6WVG3} SEQUENCE 325 AA; 35701 MW; 6254CC18E27275A9 CRC64; MALADSTRGL PNGGGGGGGS GSSSSSAEPP LFPDIVELNV GGQVYVTRRC TVVSVPDSLL WRMFTQQQPQ ELARDSKGRF FLDRDGFLFR YILDYLRDLQ LVLPDYFPER SRLQREAEYF ELPELVRRLG APQQPGPGPP PSRRGVHKEG SLGDELLPLG YSEPEQQEGA SAGAPSPTLE LASRSPSGGA AGPLLTPSQS LDGSRRSGYI TIGYRGSYTI GRDAQADAKF RRVARITVCG KTSLAKEVFG DTLNESRDPD RPPERYTSRY YLKFNFLEQA FDKLSESGFH MVACSSTGTC AFASSTDQSE DKIWTSYTEY VFCRE
Research Areas
Citations (0)

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Drenos,F.,, Hum. Mol. Genet. 18 (12), 2305-2316 (2009) Suehara,Y.,, Clin. Cancer Res. 14 (6), 1707-1717 (2008) Cauchi,S.,, J. Mol. Med. 86 (3), 341-348 (2008)

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$ 295.00
$ 99.00
Cat# AP9273c
(40 western blots)
Availability: In Stock
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