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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   DFFA Antibody (C-term)   

DFFA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DFFA Antibody (C-term) AP9286b
    Western blot analysis of DFFA (arrow) using rabbit polyclonal DFFA Antibody (C-term) (Cat. #AP9286b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DFFA gene.
  • WB - DFFA Antibody (C-term) AP9286b
    Western blot analysis of DFFA Antibody (C-term) (Cat. #AP9286b) in K562 cell line lysates (35ug/lane). DFFA (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00273
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36522 Da
Antigen Region 304-331 aa
Additional Information
Gene ID 1676
Other Names DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45
Target/Specificity This DFFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human DFFA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDFFA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms DFF1, DFF45
Function Inhibitor of the caspase-activated DNase (DFF40).
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.


Ninios,Y.P.,, Apoptosis 15 (2), 128-138 (2010)
Banas,T.,, Eur. J. Obstet. Gynecol. Reprod. Biol. 146 (1), 87-91 (2009)
Trynka,G.,, Gut 58 (8), 1078-1083 (2009)

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$ 295.00
$ 99.00
Cat# AP9286b
(40 western blots)
Availability: In Stock
Bulk Size
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