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MOUSE VGLU2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MOUSE VGLU2 Antibody (C-term) AP9300b
    All lanes : Anti-MOUSE VGLU2 Andibody (C-term) at 1:1000-1:2000 dilution Lane 1: Human brain lysate Lane 2: Human fetal brain lysate Lane 3: Human cerebellum lysate Lane 4: Mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MOUSE VGLU2 Antibody (C-term) AP9300b
    All lanes : Anti-MOUSE VGLU2 Andibody (C-term) at 1:2000 dilution Lane 1: Human fetal brain lysate Lane 2: Human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - MOUSE VGLU2 Antibody (C-term) AP9300b
    Formalin-fixed and paraffin-embedded human brain reacted with MOUSE VGLU2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - MOUSE VGLU2 Antibody (C-term) AP9300b
    Western blot analysis of MOUSE VGLU2 Andibody (C-term) (Cat. #AP9300b) in mouse cerebellum, brain tissue lysates (35ug/lane). VGLU2 (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q8BLE7
Other Accession Q9JI12, Q9P2U8, A6QLI1
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64561 Da
Antigen Region 507-535 aa
Additional Information
Gene ID 140919
Other Names Vesicular glutamate transporter 2, VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, Slc17a6, Dnpi, Vglut2
Target/Specificity This MOUSE VGLU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 507-535 amino acids from the C-terminal region of mouse VGLU2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMOUSE VGLU2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Slc17a6
Synonyms Dnpi, Vglut2
Function Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome
Tissue Location Expressed in brain. Expressed in hippocampal neurons (at protein level).
Research Areas
Citations (0)

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Background

MOUSE VGLU2 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. This protein may also mediate the transport of inorganic phosphate.

References

Birgner,C., et.al., Proc. Natl. Acad. Sci. U.S.A. 107 (1), 389-394 (2010)
Renier,N., et.al., PLoS Biol. 8 (3), E1000325 (2010)
Rose,M.F., et.al., Proc. Natl. Acad. Sci. U.S.A. 106 (52), 22462-22467 (2009)

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$ 295.00
$ 99.00
Cat# AP9300b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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