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GPX1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - GPX1 Antibody (C-term) AP9315b
    Overlay histogram showing HepG2 cells stained with AP9315b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9315b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - GPX1 Antibody (C-term) AP9315b
    Overlay histogram showing HepG2 cells stained with AP9315b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9315b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - GPX1 Antibody (C-term) AP9315b
    Overlay histogram showing HepG2 cells stained with AP9315b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9315b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - GPX1 Antibody (C-term) AP9315b
    Overlay histogram showing HepG2 cells stained with AP9315b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9315b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - GPX1 Antibody (C-term) AP9315b
    All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GPX1 Antibody (C-term) AP9315b
    All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T/17 whole cell lysate Lane 4: human kidney lysate Lane 5: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GPX1 Antibody (C-term) AP9315b
    Western blot analysis of lysates from THP-1 cell line ,mouse liver and rat liver tissue (from left to right),using GPX1 Antibody (C-term)(Cat. #AP9315b).AP9315b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • IHC-P - GPX1 Antibody (C-term) AP9315b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GPX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - GPX1 Antibody (C-term) AP9315b
    Confocal immunofluorescent analysis of GPX1 Antibody (C-term)(Cat#AP9315b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, IF, E
Primary Accession P07203
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22088 Da
Antigen Region 164-193 aa
Additional Information
Gene ID 2876
Other Names Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1
Target/Specificity This GPX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPX1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPX1
Function Protects the hemoglobin in erythrocytes from oxidative breakdown.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

GPX1 encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.

References

Moyer,A.M., et.al., Cancer Epidemiol. Biomarkers Prev. 19 (3), 811-821 (2010)
Akimoto,A.K., et.al., Free Radic. Res. 44 (3), 322-331 (2010)
Cao,C., et.al., J. Biol. Chem. 278 (41), 39609-39614 (2003)

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$ 295.00
$ 99.00
Cat# AP9315b
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Quantity:
(40 western blots)
Availability: In Stock
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