|Application ||WB, IHC-P, E|
|Calculated MW||31655 Da|
|Antigen Region||239-266 aa|
|Other Names||Centromere protein K, CENP-K, Interphase centromere complex protein 37, Protein AF-5alpha, p33, CENPK, ICEN37|
|Target/Specificity||This CENPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-266 amino acids from the C-terminal region of human CENPK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CENPK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore.|
|Cellular Location||Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex|
|Tissue Location||Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression.
Cheeseman,I.M., et.al., Mol. Biol. Cell 19 (2), 587-594 (2008)
Izuta,H., et.al., Genes Cells 11 (6), 673-684 (2006)
Okada,M., et.al., Nat. Cell Biol. 8 (5), 446-457 (2006)
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