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ATLA2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ATLA2 Antibody (Center) AP9367c
    Western blot analysis of ATLA2 Antibody (Center) (Cat. #AP9367c) in HepG2 cell line lysates (35ug/lane). ATLA2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ATLA2 Antibody (Center) AP9367c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATLA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ATLA2 Antibody (Center) AP9367c
    ATLA2 Antibody (Center) (Cat. #AP9367c) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q8NHH9
Other Accession Q6PA06, Q95LN3
Reactivity Human
Predicted Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 66229 Da
Antigen Region 297-326 aa
Additional Information
Gene ID 64225
Other Names Atlastin-2, 365-, ADP-ribosylation factor-like protein 6-interacting protein 2, ARL-6-interacting protein 2, Aip-2, ATL2, ARL6IP2
Target/Specificity This ATLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human ATLA2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATLA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATL2
Synonyms ARL6IP2
Function GTPase tethering membranes through formation of trans- homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis (PubMed:18270207, PubMed:19665976, PubMed:27619977).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Localizes at endoplasmic reticulum (ER) three-way tubular junctions (PubMed:27619977).
Tissue Location Expressed in peripheral tissues (at protein level).
Research Areas
Citations (0)
citation

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Background

ATLA2 is GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. This protein play a role in endoplasmic reticulum tubular network biogenesis.

References

Hu,J. Cell 138 (3), 549-561 (2009)
Rismanchi,N. Hum. Mol. Genet. 17 (11), 1591-1604 (2008)
Abel,A. Neurogenetics 5 (4), 239-243 (2004)

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$ 182.50
$ 70.00
Cat# AP9367c
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