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> home > Products > Primary Antibodies > Antibody Collections > Catalog Updated > ORAI1 Antibody (Center)
ORAI1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96D31 |
| Other Accession | Q5M848, Q8BWG9, Q6TLE6, Q5ZL05 |
| Reactivity | Human, Mouse |
| Predicted | Chicken, Zebrafish, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 32668 Da |
| Antigen Region | 145-173 aa |
| Gene ID | 84876 |
|---|---|
| Other Names | Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A |
| Target/Specificity | This ORAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids of human ORAI1. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ORAI1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ORAI1 |
|---|---|
| Synonyms | CRACM1, TMEM142A |
| Function | Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1 (PubMed:16582901, PubMed:16645049, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:19249086, PubMed:23307288, PubMed:24351972, PubMed:24591628, PubMed:28219928, PubMed:20354224, PubMed:26956484). CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT (PubMed:16582901). Plays a prominent role in Ca(2+) influx at the basolateral membrane of mammary epithelial cells independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca(2+) for milk secretion. |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q8BWG9}; Multi-pass membrane protein. Note=Isoform beta is more mobile in the plasma membrane (PubMed:23307288). Colocalizes with STIM1 at the cell membrane (PubMed:27185316). |
| Tissue Location | Expressed in naive CD4 and CD8 T cells (at protein level) (PubMed:26956484). Expressed at similar levels in naive and effector T helper cells (PubMed:20354224) |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
ORAI1 is a plasma membrane protein essential for store-operated calcium entry.
References
Zhou,Y. Proc. Natl. Acad. Sci. U.S.A. 107 (11), 4896-4901 (2010)
Calloway,N. Biochemistry 49 (6), 1067-1071 (2010)
McCarl,C.A. J. Allergy Clin. Immunol. 124 (6), 1311-1318 (2009)
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Anonymous
2016-08-23 21:15:32
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Species tested
Human,Mouse,Rat
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
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$ 365.00
$ 140.00
Cat# AP9371c
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