|Application ||WB, IHC-P, E|
|Other Accession||Q5M848, Q8BWG9, Q6TLE6, Q5ZL05|
|Predicted||Rat, Zebrafish, Chicken|
|Calculated MW||32668 Da|
|Antigen Region||145-173 aa|
|Other Names||Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A|
|Target/Specificity||This ORAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the Central region of human ORAI1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ORAI1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.|
|Cellular Location||Cell membrane; Multi- pass membrane protein Note=Isoform beta is more mobile in the plasma membrane|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
ORAI1 is a plasma membrane protein essential for store-operated calcium entry.
Zhou,Y. Proc. Natl. Acad. Sci. U.S.A. 107 (11), 4896-4901 (2010)
Calloway,N. Biochemistry 49 (6), 1067-1071 (2010)
McCarl,C.A. J. Allergy Clin. Immunol. 124 (6), 1311-1318 (2009)
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1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose