|Application ||FC, IHC-P, WB, E|
|Calculated MW||70178 Da|
|Antigen Region||49-79 aa|
|Other Names||Peroxisomal carnitine O-octanoyltransferase, COT, CROT, COT|
|Target/Specificity||This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CROT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
Li, Y.L., et al. FEBS J. 276(1):303-314(2009)
Morillas, M., et al. J. Biol. Chem. 277(13):11473-11480(2002)
van der Leij, F.R., et al. Mol. Genet. Metab. 71 (1-2), 139-153 (2000) :
Ferdinandusse, S., et al. Biochem. Biophys. Res. Commun. 263(1):213-218(1999)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.