|Application ||FC, IHC-P, WB, E|
|Calculated MW||70178 Da|
|Antigen Region||49-79 aa|
|Other Names||Peroxisomal carnitine O-octanoyltransferase, COT, CROT, COT|
|Target/Specificity||This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CROT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl- CoA, to its corresponding carnitine ester.|
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Provided below are standard protocols that you may find useful for product applications.
CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
Li, Y.L., et al. FEBS J. 276(1):303-314(2009)
Morillas, M., et al. J. Biol. Chem. 277(13):11473-11480(2002)
van der Leij, F.R., et al. Mol. Genet. Metab. 71 (1-2), 139-153 (2000) :
Ferdinandusse, S., et al. Biochem. Biophys. Res. Commun. 263(1):213-218(1999)
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