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>   home   >   Products   >   Primary Antibodies   >   CXXC4 Antibody (C-term)   

CXXC4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CXXC4 Antibody (C-term) AP9385b
    Western blot analysis of CXXC4 Antibody (C-term) (Cat. #AP9385b) in mouse testis tissue lysates (35ug/lane). CXXC4 (arrow) was detected using the purified Pab.
  • IHC-P - CXXC4 Antibody (C-term) AP9385b
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CXXC4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CXXC4 Antibody (C-term) AP9385b
    CXXC4 Antibody (C-term) (Cat. #AP9385b) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - CXXC4 Antibody (C-term) AP9385b
    CXXC4 Antibody (C-term) (Cat. #AP9385b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CXXC4 antibody detected the CXXC4 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9H2H0
Other Accession Q9EQC9, Q6NXI8
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 158-185 aa
Additional Information
Other Names CXXC-type zinc finger protein 4, Inhibition of the Dvl and axin complex protein, CXXC4, IDAX
Target/Specificity This CXXC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-185 amino acids from the C-terminal region of human CXXC4.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCXXC4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CXXC4
Synonyms IDAX
Function Acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.
Cellular Location Cytoplasm. EMBL; AF272159; AAG42072.1; -; mRNA EMBL; AC093628; AAY40933.1; -; Genomic_DNA EMBL; CH471057; EAX06175.1; -; Genomic_DNA EMBL; BC119751; AAI19752.1; -; mRNA EMBL; BC119752; AAI19753.1; -; mRNA UniGene; Hs.12248; - UniGene; Hs.624057; - PDB; 5VC9; X-ray; 2.10 A; C/F=133-180 PDBsum; 5VC9; - ProteinModelPortal; Q9H2H0; - SMR; Q9H2H0; - IntAct; Q9H2H0; 6 STRING; 9606.ENSP00000412267; - iPTMnet; Q9H2H0; - PhosphoSitePlus; Q9H2H0; - DMDM; 74752605; - PaxDb; Q9H2H0; - PRIDE; Q9H2H0; - ProteomicsDB; 80549; - Ensembl; ENST00000426831; ENSP00000412267; ENSG00000168772 DisGeNET; 80319; - GeneCards; CXXC4; - HGNC; HGNC:24593; CXXC4 HPA; HPA036600; - MIM; 611645; gene neXtProt; NX_Q9H2H0; - PharmGKB; PA134992016; - eggNOG; ENOG410IEZT; Eukaryota eggNOG; ENOG4111BB0; LUCA HOGENOM; HOG000112118; - HOVERGEN; HBG107791; - InParanoid; Q9H2H0; - PhylomeDB; Q9H2H0; - TreeFam; TF326617; - Reactome; R-HSA-5368598; Negative regulation of TCF-dependent signaling by DVL-interacting proteins PRO; PR:Q9H2H0; - Proteomes; UP000005640; Unplaced CleanEx; HS_CXXC4; - GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL GO; GO:0031410; C:cytoplasmic vesicle; IDA:BHF-UCL GO; GO:0003677; F:DNA binding; IEA:InterPro GO; GO:0030165; F:PDZ domain binding; IPI:BHF-UCL GO; GO:0008270; F:zinc ion binding; IEA:InterPro GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IDA:BHF-UCL GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW GO; GO:0007352; P:zygotic specification of dorsal/ventral axis; ISS:BHF-UCL InterPro; IPR002857; Znf_CXXC Pfam; PF02008; zf-CXXC; 1 PROSITE; PS51058; ZF_CXXC; 1 1: Evidence at protein level; 3D-structure; Complete proteome; Cytoplasm; Metal-binding; Reference proteome; Wnt signaling pathway; Zinc; Zinc-finger CHAIN 1 198 CXXC-type zinc finger protein 4 /FTId=PRO_0000317542 ZN_FING 132 173 CXXC-type. {ECO:0000255|PROSITE- ProRule:PRU00509} REGION 161 166 Interaction with DVL1. COMPBIAS 116 123 Poly-Ser HELIX 143 146 {ECO:0000244|PDB:5VC9} HELIX 155 158 {ECO:0000244|PDB:5VC9} HELIX 160 163 {ECO:0000244|PDB:5VC9} TURN 168 170 {ECO:0000244|PDB:5VC9} HELIX 173 175 {ECO:0000244|PDB:5VC9} SEQUENCE 198 AA; 20978 MW; 9D9F7FAD11A9B759 CRC64; MHHRNDSQRL GKAGCPPEPS LQMANTNFLS TLSPEHCRPL AGECMNKLKC GAAEAEIMNL PERVGTFSAI PALGGISLPP GVIVMTALHS PAAASAAVTD SAFQIANLAD CPQNHSSSSS SSSGGAGGAN PAKKKRKRCG VCVPCKRLIN CGVCSSCRNR KTGHQICKFR KCEELKKKPG TSLERTPVPS AEAFRWFF
Citations (0)

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Background

CXXC4 acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.

References

Kojima, T., et al. Oncogene 28(2):297-305(2009)
Hino, S., et al. Mol. Cell. Biol. 21(1):330-342(2001)

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Cat# AP9385b
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