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EXT2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - EXT2 Antibody (Center) AP9401c
    Western blot analysis of lysate from T47D cell line,using EXT2 Antibody (Center)(Cat. #AP9401c).AP9401c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
  • IHC-P - EXT2 Antibody (Center) AP9401c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - EXT2 Antibody (Center) AP9401c
    Confocal immunofluorescent analysis of EXT2 Antibody (Center)(Cat#AP9401c) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - EXT2 Antibody (Center) AP9401c
    EXT2 Antibody (Center) (Cat. #AP9401c) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q93063
Other Accession P70428, O77783
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82255 Da
Antigen Region 182-209 aa
Additional Information
Gene ID 2132
Other Names Exostosin-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Multiple exostoses protein 2, Putative tumor suppressor protein EXT2, EXT2
Target/Specificity This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-209 amino acids from the Central region of human EXT2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEXT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EXT2
Function Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor.
Cellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note=The EXT1/EXT2 complex is localized in the Golgi apparatus
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.


Zhao, J., et al. Diabetes 59(3):751-755(2010)
Li, Y., et al. Genet Test Mol Biomarkers 13(6):825-830(2009)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Heinritz, W., et al. Ann. Hum. Genet. 73 (PT 3), 283-291 (2009)
Cornelis, M.C., et al. Ann. Intern. Med. 150(8):541-550(2009)

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$ 295.00
$ 99.00
Cat# AP9401c
(40 western blots)
Availability: In Stock
Bulk Size
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