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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   p19 Antibody (N-term)   

p19 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - p19 Antibody (N-term) AP9406A
    Anti-p19 Antibody (N-term) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - p19 Antibody (N-term) AP9406A
    Western blot analysis of p19 Antibody (N-term) (Cat. #AP9406a) in mouse testis tissue lysates (35ug/lane). p19 (arrow) was detected using the purified Pab.
  • IHC-P - p19 Antibody (N-term) AP9406A
    p19 Antibody (N-term)(Cat. #AP9406a) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the p19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - p19 Antibody (N-term) AP9406A
    p19 Antibody (N-term) (Cat. #AP9406a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P55273
Other Accession Q60773
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 17700 Da
Antigen Region 15-43 aa
Additional Information
Gene ID 1032
Other Names Cyclin-dependent kinase 4 inhibitor D, p19-INK4d, CDKN2D
Target/Specificity This p19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human p19.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp19 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN2D
Function Interacts strongly with CDK4 and CDK6 and inhibits them.
Cellular Location Nucleus. Cytoplasm
Research Areas
Citations (0)

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Background

p19 is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis.

References

Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Zhang, W., et al. Acta Biochim. Biophys. Sin. (Shanghai) 41(5):414-428(2009)
Agarwal, S.K., et al. J. Clin. Endocrinol. Metab. 94(5):1826-1834(2009)
Goode, E.L., et al. Cancer Epidemiol. Biomarkers Prev. 18(3):935-944(2009)
Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009)

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Cat# AP9406A
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(40 western blots)
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