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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   FBXL5 Antibody (N-term)   

FBXL5 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - FBXL5 Antibody (N-term) AP9409a
    Western blot analysis of FBXL5 Antibody (N-term) (Cat. #AP9409a) in HepG2 cell line lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab;
  • WB - FBXL5 Antibody (N-term) AP9409a
    Western blot analysis of FBXL5 Antibody (N-term) (Cat. #AP9409a) in mouse heart tissue lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab.
  • IHC-P - FBXL5 Antibody (N-term) AP9409a
    FBXL5 Antibody (N-term) (Cat. #AP9409a) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - FBXL5 Antibody (N-term) AP9409a
    FBXL5 Antibody (N-term) (Cat. #AP9409a) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UKA1
Other Accession Q8C2S5, A2VE78
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 78555 Da
Antigen Region 86-115 aa
Additional Information
Gene ID 26234
Other Names F-box/LRR-repeat protein 5, F-box and leucine-rich repeat protein 5, F-box protein FBL4/FBL5, p45SKP2-like protein, FBXL5, FBL4, FBL5, FLR1
Target/Specificity This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human FBXL5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFBXL5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FBXL5
Synonyms FBL4, FBL5, FLR1
Function Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.
Cellular Location Cytoplasm, perinuclear region
Research Areas
Citations (0)

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Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
Vashisht, A.A., et al. Science 326(5953):718-721(2009)
Zhang, N., et al. Biochem. Biophys. Res. Commun. 359(1):34-39(2007)
Ilyin, G.P., et al. Genomics 67(1):40-47(2000)
Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)
Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999)

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$ 295.00
$ 99.00
Cat# AP9409a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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