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> home > Products > Primary Antibodies > Antibody Collections > Catalog Updated > FBXL5 Antibody (N-term)
FBXL5 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q9UKA1 |
| Other Accession | Q8C2S5, A2VE78 |
| Reactivity | Human, Mouse |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 78555 Da |
| Antigen Region | 86-115 aa |
| Gene ID | 26234 |
|---|---|
| Other Names | F-box/LRR-repeat protein 5, F-box and leucine-rich repeat protein 5, F-box protein FBL4/FBL5, p45SKP2-like protein, FBXL5, FBL4, FBL5, FLR1 |
| Target/Specificity | This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human FBXL5. |
| Dilution | WB~~1:1000 IHC-P~~1:100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | FBXL5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FBXL5 |
|---|---|
| Synonyms | FBL4, FBL5, FLR1 |
| Function | Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2 (PubMed:19762596, PubMed:19762597). The C-terminal domain of FBXL5 contains a redox-sensitive [2Fe-2S] cluster that, upon oxidation, promotes binding to IRP2 to effect its oxygen-dependent degradation (PubMed:32126207). Under iron deficiency conditions, the N-terminal hemerythrin-like (Hr) region, which contains a diiron metal center, cannot bind iron and undergoes conformational changes that destabilize the FBXL5 protein and cause its ubiquitination and degradation (PubMed:19762596, PubMed:19762597). When intracellular iron levels start rising, the Hr region is stabilized (PubMed:19762596, PubMed:19762597). Additional increases in iron levels facilitate the assembly and incorporation of a redox active [2Fe-2S] cluster in the C- terminal domain (PubMed:32126207). Only when oxygen level is high enough to maintain the cluster in its oxidized state can FBXL5 recruit IRP2 as a substrate for polyubiquination and degradation (PubMed:32126207). Promotes ubiquitination and subsequent degradation of the dynactin complex component DCTN1 (PubMed:17532294). Within the nucleus, promotes the ubiquitination of SNAI1; preventing its interaction with DNA and promoting its degradation (PubMed:24157836). Negatively regulates DNA damage response by mediating the ubiquitin- proteasome degradation of the DNA repair protein NABP2 (PubMed:25249620). |
| Cellular Location | Cytoplasm, perinuclear region. Nucleus |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
Vashisht, A.A., et al. Science 326(5953):718-721(2009)
Zhang, N., et al. Biochem. Biophys. Res. Commun. 359(1):34-39(2007)
Ilyin, G.P., et al. Genomics 67(1):40-47(2000)
Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)
Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999)
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$ 365.00
$ 140.00
Cat# AP9409a
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