|Application ||WB, E|
|Calculated MW||68265 Da|
|Antigen Region||516-545 aa|
|Other Names||RNA polymerase II elongation factor ELL, Eleven-nineteen lysine-rich leukemia protein, ELL, C19orf17|
|Target/Specificity||This ELL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human ELL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ELL Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.|
|Cellular Location||Nucleus. Nucleus speckle Nucleus, Cajal body Note=Colocalizes with EAF2 to nuclear speckles (PubMed:12446457) Colocalizes with coilin in subnuclear cajal and histone locus bodies (PubMed:12686606). Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes(PubMed:23932780).|
|Tissue Location||Expressed in all tissues tested. Highest levels found in placenta, skeletal muscle, testis and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.
?Zhou, J., et al. J. Biol. Chem. 284(28):19142-19152(2009)
?Jiang, F., et al. Cancer Lett. 253(2):265-272(2007)
?Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
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