|Application ||WB, E|
|Calculated MW||102884 Da|
|Antigen Region||60-89 aa|
|Other Names||Flap endonuclease GEN homolog 1, 31--, GEN1|
|Target/Specificity||This GEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human GEN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GEN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation.|
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Provided below are standard protocols that you may find useful for product applications.
Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded (By similarity).
Ip, S.C., et al. Nature 456(7220):357-361(2008)
Ishikawa, G., et al. Nucleic Acids Res. 32(21):6251-6259(2004)
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