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ADD1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP9518c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP9518c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
ADD1 Antibody (Center) - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | P35611 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 80955 Da |
ADD1 Antibody (Center) - Additional info | |
| Gene ID 118 | |
| Other Names ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha | |
| Target/Specificity This ADD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573~603 amino acids from the Central region of human ADD1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ADD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ADD1 Antibody (Center) - Protein Information | |
| Name ADD1 | |
| Synonyms ADDA | |
| Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin | |
| Cellular Location Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side | |
| Tissue Location Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit | |
ADD1 Antibody (Center) - Related products
AP13986b: ADD1 Antibody (C-term)
AP9518c: ADD1 Antibody (Center)
DC03630: Human ADD1 cDNA Clone
LY12219a: ADD1 Over-expression Lysate
BP9518c: ADD1 Antibody (Center) Blocking Peptide
AT1053a: ADD1 Antibody (monoclonal) (M01)
AJ1015a: Adducin alpha Antibody (C-term)
AJ1015b: Adducin alpha Antibody Phospho (pS481)
ADD1 Antibody (Center) - Application data
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Western blot analysis of ADD1 Antibody (Center) (Cat. #AP9518c) in A2058 cell line lysates (35ug/lane). ADD1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ADD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of ADD1 Antibody (Center) (Cat#AP9518c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ADD1 Antibody (Center) - Research Areas
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BACKGROUND
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C.
REFERENCES
Davila, S., et al. Genes Immun. (2010) In press : Paternoster, L., et al. Circ Cardiovasc Genet 3(1):15-21(2010) Ferrandi, M., et al. J. Mol. Med. 88(2):203-217(2010) Wang, R., et al. J. Appl. Genet. 51(1):87-94(2010) Niu, W.Q., et al. J Hum Hypertens (2009) In press : Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
