|Application ||WB, IHC-P, FC, E|
|Calculated MW||64990 Da|
|Antigen Region||437-465 aa|
|Other Names||Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A, Eukaryotic translation initiation factor 2A, N-terminally processed, EIF2A|
|Target/Specificity||This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-465 amino acids from the Central region of human EIF2A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF2A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.|
|Tissue Location||Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).
Willis, K.L., et al. Virology 394(1):73-81(2009)
Spurgeon, M.E., et al. J. Virol. 83(19):9970-9982(2009)
Lu, W., et al. J. Biol. Chem. 284(36):24281-24288(2009)
Yang, J., et al. Mol. Cell. Biol. 29(8):2243-2253(2009)
Schewe, D.M., et al. Cancer Res. 69(4):1545-1552(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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