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EIF2A Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EIF2A Antibody (Center) AP9521c
    Western blot analysis of EIF2A Antibody (Center) (Cat. #AP9521c) in Ramos cell line lysates (35ug/lane). EIF2A (arrow) was detected using the purified Pab.
  • IHC-P - EIF2A Antibody (Center) AP9521c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EIF2A Antibody (Center) AP9521c
    EIF2A Antibody (Center) (Cat. #AP9521c) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BY44
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64990 Da
Antigen Region 437-465 aa
Additional Information
Gene ID 83939
Other Names Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A, Eukaryotic translation initiation factor 2A, N-terminally processed, EIF2A
Target/Specificity This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-465 amino acids from the Central region of human EIF2A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF2A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2A
Function Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
Tissue Location Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta
Citations (0)

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EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).


Willis, K.L., et al. Virology 394(1):73-81(2009)
Spurgeon, M.E., et al. J. Virol. 83(19):9970-9982(2009)
Lu, W., et al. J. Biol. Chem. 284(36):24281-24288(2009)
Yang, J., et al. Mol. Cell. Biol. 29(8):2243-2253(2009)
Schewe, D.M., et al. Cancer Res. 69(4):1545-1552(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 295.00
$ 99.00
Cat# AP9521c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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