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NUCKS1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NUCKS1 Antibody (N-term) AP9527a
    Western blot analysis of NUCKS1 Antibody (N-term) (Cat. #AP9527a) in Ramos cell line lysates (35ug/lane). NUCKS1 (arrow) was detected using the purified Pab.
    detail
  • FC - NUCKS1 Antibody (N-term) AP9527a
    NUCKS1 Antibody (N-term) (Cat. #AP9527a) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9H1E3
Other Accession NP_073568
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27296 Da
Antigen Region 45-73 aa
Additional Information
Gene ID 64710
Other Names Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1, P1, NUCKS1, NUCKS
Target/Specificity This NUCKS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-73 amino acids from the N-terminal region of human NUCKS1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUCKS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUCKS1 {ECO:0000303|PubMed:26323318, ECO:0000312|HGNC:HGNC:29923}
Function Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR) (PubMed:26323318). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D- loop structures, but with less affinity than RAD51AP1 (PubMed:26323318).
Cellular Location Nucleus. Chromosome
Tissue Location Widely expressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung
Research Areas
Citations (0)
citation

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References

Satake, W., et al. Nat. Genet. 41(12):1303-1307(2009)
Simon-Sanchez, J., et al. Nat. Genet. 41(12):1308-1312(2009)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Wisniewski, J.R., et al. Proteins 73(3):710-718(2008)
Grundt, K., et al. Biochim. Biophys. Acta 1773(9):1398-1406(2007)

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$ 365.00
$ 140.00
Cat# AP9527a
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