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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CHMP4B Antibody (N-term)   

CHMP4B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CHMP4B Antibody (N-term) AP9528a
    Western blot analysis of CHMP4B Antibody (N-term) (Cat. #AP9528a) in SK-BR-3 cell line lysates (35ug/lane).CHMP4B (arrow) was detected using the purified Pab.
  • IHC-P - CHMP4B Antibody (N-term) AP9528a
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CHMP4B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CHMP4B Antibody (N-term) AP9528a
    CHMP4B Antibody (N-term) (Cat. #AP9528a) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H444
Other Accession P59074, Q6IQ73, Q5XGW6, Q9D8B3, Q7ZVC4, Q5ZHP5, NP_789782
Reactivity Human
Predicted Chicken, Zebrafish, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24950 Da
Antigen Region 51-79 aa
Additional Information
Gene ID 128866
Other Names Charged multivesicular body protein 4b, Chromatin-modifying protein 4b, CHMP4b, SNF7 homolog associated with Alix 1, SNF7-2, hSnf7-2, Vacuolar protein sorting-associated protein 32-2, Vps32-2, hVps32-2, CHMP4B, C20orf178, SHAX1
Target/Specificity This CHMP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human CHMP4B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHMP4B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms C20orf178, SHAX1
Function Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.
Cellular Location Cytoplasm, cytosol. Late endosome membrane; Peripheral membrane protein
Tissue Location Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
Research Areas
Citations ( 0 )


CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.


Zhou, X., et al. Biochem. J. 418(2):277-284(2009)
McCullough, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(22):7687-7691(2008)
Ichioka, F., et al. FEBS J. 275(4):682-692(2008)
Hanson, P.I., et al. J. Cell Biol. 180(2):389-402(2008)
Shiels, A., et al. Am. J. Hum. Genet. 81(3):596-606(2007)

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$ 295.00
$ 99.00
Cat# AP9528a
(40 western blots)
Availability: In Stock
Bulk Size
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