|Application ||WB, E|
|Other Accession||Q6AY57, Q80W47, Q5ZHN3|
|Calculated MW||49408 Da|
|Antigen Region||426-454 aa|
|Other Names||WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2, WIPI2|
|Target/Specificity||This WIPI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-454 amino acids from the C-terminal region of human WIPI2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||WIPI2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.|
|Cellular Location||Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.|
|Tissue Location||Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas Expression is down-regulated in pancreatic and in kidney tumors|
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Provided below are standard protocols that you may find useful for product applications.
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).
Proikas-Cezanne, T., et al. Oncogene 23(58):9314-9325(2004)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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