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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   CHAT Antibody (N-term)   

CHAT Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CHAT Antibody (N-term) AP9593a
    Western blot analysis of CHAT Antibody (N-term) (Cat. #AP9593a) in NCI-H460 cell line lysates (35ug/lane).CHAT (arrow) was detected using the purified Pab.
  • WB - CHAT Antibody (N-term) AP9593a
    Western blot analysis of CHAT Antibody (N-term) (Cat. #AP9593a) in mouse liver tissue lysates (35ug/lane). CHAT (arrow) was detected using the purified Pab.
  • IF - CHAT Antibody (N-term) AP9593a
    Confocal immunofluorescent analysis of CHAT Antibody (N-term) (Cat. #AP9593a) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28329
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82536 Da
Antigen Region 98-128 aa
Additional Information
Gene ID 1103
Other Names Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
Target/Specificity This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHAT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Research Areas
Citations (0)

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CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.


Shaffer, J.R., et al. Nutr Metab Cardiovasc Dis (2010) In press
Beck, M., et al. Cell Tissue Res. 338(1):37-51(2009)
Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)

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$ 295.00
$ 99.00
Cat# AP9593a
(40 western blots)
Availability: In Stock
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