MICAL2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O94851 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 219061 Da |
Antigen Region | 55-83 aa |
Gene ID | 9645 |
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Other Names | Protein-methionine sulfoxide oxidase MICAL2, 11413-, Molecule interacting with CasL protein 2, MICAL-2, MICAL2, KIAA0750, MICAL2PV1, MICAL2PV2 |
Target/Specificity | This MICAL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the N-terminal region of human MICAL2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MICAL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MICAL2 (HGNC:24693) |
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Function | Methionine monooxygenase that promotes depolymerization of F- actin by mediating oxidation of residues 'Met-44' and 'Met-47' on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:24440334, PubMed:29343822). Regulates the disassembly of branched actin networks also by oxidizing ARP3B-containing ARP2/3 complexes leading to ARP3B dissociation from the network (PubMed:34106209). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF- A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (PubMed:24440334). |
Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q8BML1} |
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Provided below are standard protocols that you may find useful for product applications.
References
Ashida, S., et al. Clin. Cancer Res. 12(9):2767-2773(2006)
Fischer, J., et al. Biochem. Biophys. Res. Commun. 328(2):415-423(2005)
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