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B3GNT5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - B3GNT5 Antibody (Center) AP9649c
    B3GNT5 Antibody (Center) (Cat. #AP9649c) western blot analysis in 293,MDA-MB435,HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT5 antibody detected the B3GNT5 protein (arrow).
  • WB - B3GNT5 Antibody (Center) AP9649c
    Western blot analysis of B3GNT5 Antibody (Center) (Cat. #AP9649c) in mouse liver tissue lysates (35ug/lane). B3GNT5 (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BYG0
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44053 Da
Antigen Region 118-145 aa
Additional Information
Gene ID 84002
Other Names Lactosylceramide 1, 3-N-acetyl-beta-D-glucosaminyltransferase, Lactotriaosylceramide synthase, Lc(3)Cer synthase, Lc3 synthase, UDP-GlcNAc:beta-Gal beta-1, 3-N-acetylglucosaminyltransferase 5, BGnT-5, Beta-1, 3-Gn-T5, Beta-1, 3-N-acetylglucosaminyltransferase 5, Beta3Gn-T5, B3GNT5
Target/Specificity This B3GNT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human B3GNT5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB3GNT5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B3GNT5
Function Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus
Citations (0)

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B3GNT5 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids.


Marcos, N.T., et al. J. Clin. Invest. 118(6):2325-2336(2008)
Togayachi, A., et al. J. Biol. Chem. 276(25):22032-22040(2001)

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$ 295.00
$ 99.00
Cat# AP9649c
(40 western blots)
Availability: In Stock
Bulk Size
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