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TLR4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TLR4 Antibody (Center) AP9685c
    Western blot analysis of TLR4 Antibody (Center) (Cat. #AP9685c) in HL-60 cell line lysates (35ug/lane). TLR4 (arrow) was detected using the purified Pab.
  • FC - TLR4 Antibody (Center) AP9685c
    TLR4 Antibody (Center) (Cat. #AP9685c) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00206
Other Accession Q68Y56, Q9WV82, Q9GL65, Q9MYW3
Reactivity Human
Predicted Bovine, Hamster, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 95680 Da
Antigen Region 669-698 aa
Additional Information
Gene ID 7099
Other Names Toll-like receptor 4, hToll, CD284, TLR4
Target/Specificity This TLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-698 amino acids from the Central region of human TLR4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTLR4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TLR4
Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species- specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis
Tissue Location Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells
Research Areas
Citations (0)

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TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.


# Sam-Agudu, N.A., et al. Am. J. Trop. Med. Hyg. 82(4):548-555(2010)
# Palomino-Morales, R.J., et al. Arthritis Res. Ther. 12 (2), R51 (2010)
# Rigoli, L., et al. Anticancer Res. 30(2):513-517(2010)
# da Silva Correia, J., et al. J. Biol. Chem. 277(3):1845-1854(2002)
# Shimazu, R., et al. J. Exp. Med. 189(11):1777-1782(1999)

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$ 295.00
$ 99.00
Cat# AP9685c
(40 western blots)
Availability: In Stock
Bulk Size
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