INSC Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q1MX18 |
Other Accession | Q3HNM7 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 63469 Da |
Antigen Region | 410-437 aa |
Gene ID | 387755 |
---|---|
Other Names | Protein inscuteable homolog, INSC |
Target/Specificity | This INSC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-437 amino acids from the Central region of human INSC. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | INSC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | INSC |
---|---|
Function | May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex (PubMed:16458856). Involved in spindle orientation during mitosis. May regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium (By similarity). |
Cellular Location | Cytoplasm. Cytoplasm, cell cortex. Note=Uniformly distributed in the cytoplasm during interphase. During metaphase, detected in the cell cortex, adjacent to the mitotic spindle poles |
Tissue Location | Isoform 1 is expressed in various tissues with stronger expression in liver, kidney and small intestine. Isoform 2 is abundantly expressed in small intestine and to a lower extent in lung and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
In Drosophila, neuroblasts divide asymmetrically into another neuroblast at the apical side and a smaller ganglion mother cell on the basal side. Cell polarization is precisely regulated by 2 apically localized multiprotein signaling complexes that are tethered by Inscuteable, which regulates their apical localization (Izaki et al., 2006 [PubMed 16458856]).
References
Vural, A., et al. Mol. Cell. Biol. 30(6):1528-1540(2010)
Rivadeneira, F., et al. Nat. Genet. 41(11):1199-1206(2009)
Izaki, T., et al. Biochem. Biophys. Res. Commun. 341(4):1001-1006(2006)
Katoh, M., et al. Int. J. Mol. Med. 11(1):111-116(2003)
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