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ZDHHC9 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ZDHHC9 Antibody (C-term) AP9716b
    Western blot analysis of ZDHHC9 Antibody (C-term) (Cat. #AP9716b) in mouse liver tissue lysates (35ug/lane). ZDHHC9 (arrow) was detected using the purified Pab.
    detail
  • WB - ZDHHC9 Antibody (C-term) AP9716b
    Anti-ZDHHC9 Antibody (C-term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ZDHHC9 Antibody (C-term) AP9716b
    Anti-ZDHHC9 Antibody (C-term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ZDHHC9 Antibody (C-term) AP9716b
    ZDHHC9 Antibody (C-term) (Cat. #AP9716b) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZDHHC9 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y397
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40916 Da
Antigen Region 335-364 aa
Additional Information
Gene ID 51114
Other Names Palmitoyltransferase ZDHHC9, Zinc finger DHHC domain-containing protein 9, DHHC-9, DHHC9, Zinc finger protein 379, Zinc finger protein 380, ZDHHC9, CXorf11, ZDHHC10, ZNF379, ZNF380
Target/Specificity This ZDHHC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-364 amino acids from the C-terminal region of human ZDHHC9.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZDHHC9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZDHHC9
Synonyms CXorf11, ZDHHC10, ZNF379, ZNF380
Function Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates (Probable). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS (PubMed:16000296). May have a palmitoyltransferase activity toward the beta-2 adrenergic receptor/ADRB2 and therefore regulate G protein- coupled receptor signaling (PubMed:27481942).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein
Tissue Location Highly expressed in kidney, skeletal muscle, brain, lung and liver. Absent in thymus, spleen and leukocytes
Research Areas
Citations (0)
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Background

The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

References

Mansilla, F., et al. Br. J. Cancer 96(12):1896-1903(2007)
Raymond, F.L., et al. Am. J. Hum. Genet. 80(5):982-987(2007)
Zhou, F.L., et al. Cancer Immunol. Immunother. 55(8):910-917(2006)

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$ 365.00
$ 140.00
Cat# AP9716b
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