|Application ||WB, IHC-P, FC, E|
|Calculated MW||50200 Da|
|Antigen Region||360-389 aa|
|Other Names||Protein prune homolog, hPrune, Drosophila-related expressed sequence 17, DRES-17, DRES17, HTcD37, PRUNE|
|Target/Specificity||This PRUNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids from the C-terminal region of human PRUNE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRUNE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis (PubMed:28334956). Involved in the regulation of microtubule polymerization (PubMed:28334956).|
|Cellular Location||Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed|
|Tissue Location||Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. It plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Middelhaufe, S., et al. Biochem. J. 407(2):199-205(2007)
Kobayashi, T., et al. Mol. Cell. Biol. 26(3):898-911(2006)
Zollo, M., et al. Clin. Cancer Res. 11(1):199-205(2005)
Forus, A., et al. Oncogene 20(47):6881-6890(2001)
Reymond, A., et al. Oncogene 18(51):7244-7252(1999)
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