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PIGN Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PIGN Antibody (N-term) AP9751a
    Western blot analysis of PIGN Antibody (N-term) (Cat. #AP9751a) in mouse NIH-3T3 cell line lysates (35ug/lane). PIGN (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95427
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 105810 Da
Antigen Region 206-235 aa
Additional Information
Gene ID 23556
Other Names GPI ethanolamine phosphate transferase 1, 2---, MCD4 homolog, Phosphatidylinositol-glycan biosynthesis class N protein, PIG-N, PIGN, MCD4
Target/Specificity This PIGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-235 amino acids from the N-terminal region of human PIGN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIGN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MCD4
Function Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor (By similarity). May act as suppressor of replication stress and chromosome missegregation.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Citations (0)

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PIGN is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor.


McDonough, C.W., et al. Hum. Genet. (2009) In press :
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Kinoshita, T., et al. Curr Opin Chem Biol 4(6):632-638(2000)
Hong, Y., et al. J. Biol. Chem. 274(49):35099-35106(1999)
Gaynor, E.C., et al. Mol. Biol. Cell 10(3):627-648(1999)

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Cat# AP9751a
(40 western blots)
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