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ST8SIA4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ST8SIA4 Antibody (Center) AP9771C
    Western blot analysis of ST8SIA4 Antibody (Center) (Cat. #AP9771c) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) (Cat. #AP9771c) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.
  • IHC-P - ST8SIA4 Antibody (Center) AP9771C
    ST8SIA4 Antibody (Center) (Cat. #AP9771c) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ST8SIA4 Antibody (Center) AP9771C
    ST8SIA4 Antibody (Center) (Cat. #AP9771c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92187
Other Accession Q64692, Q6ZXC9
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41295 Da
Antigen Region 186-214 aa
Additional Information
Gene ID 7903
Other Names CMP-N-acetylneuraminate-poly-alpha-2, 8-sialyltransferase, 2499-, Alpha-2, 8-sialyltransferase 8D, Polysialyltransferase-1, Sialyltransferase 8D, SIAT8-D, Sialyltransferase St8Sia IV, ST8SiaIV, ST8SIA4, PST, PST1, SIAT8D
Target/Specificity This ST8SIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ST8SIA4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsST8SIA4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ST8SIA4
Synonyms PST, PST1, SIAT8D
Function Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Highly expressed in fetal brain, lung and kidney and in adult heart, spleen and thymus. Present to a lesser extent in adult brain, placenta, lung, large and small intestine and peripheral blood leukocytes
Citations (0)

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The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus.


Johnson, M.P., et al. Hum. Genet. 126(5):655-666(2009)
Foley, D.A., et al. J. Biol. Chem. 284(23):15505-15516(2009)
Otowa, T., et al. J. Hum. Genet. 54(2):122-126(2009)
Sonuga-Barke, E.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1359-1368 (2008) :
Schreiber, S.C., et al. Gastroenterology 134(5):1555-1566(2008)
Close, B.E., et al. Glycobiology 11(11):997-1008(2001)
Angata, K., et al. J. Biol. Chem. 276(18):15369-15377(2001)

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Cat# AP9771C
(40 western blots)
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